Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 104.974, 157.379, 182.635 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 87.330 - 3.250 |
| R-factor | 0.2087 |
| Rwork | 0.205 |
| R-free | 0.28620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6d88 |
| RMSD bond angle | 17.891 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21_5207: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 182.630 | 3.420 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Number of reflections | 43659 | 6974 |
| <I/σ(I)> | 11.7 | |
| Completeness [%] | 89.8 | |
| Redundancy | 10.8 | |
| CC(1/2) | 0.558 | 0.891 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 6% PEG 4000, 5% Glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2 |
