8YRK

Tubulin-Compound KY216: stathmin-like domain complex

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	104.097, 158.982, 179.057
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.540 - 2.740
R-factor	0.1754
Rwork	0.173
R-free	0.22610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1sa1
RMSD bond length	0.004
RMSD bond angle	0.675
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	FRFS
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.540	2.838
High resolution limit [Å]	2.740	2.740
Rmerge	0.153
Rmeas	0.161
Rpim	0.048
Number of reflections	75501	7561
<I/σ(I)>	11.57
Completeness [%]	95.9
Redundancy	10.2
CC(1/2)		0.651

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	PEG,magnesium choride,calcium chloride,Ethylene glycol,MES