8YP1

Crystal structure of HARS WHEP domain fused with Fc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	59.943, 112.984, 110.211
Unit cell angles	90.00, 90.37, 90.00

Refinement procedure

Resolution	49.580 - 2.790
R-factor	0.21869
Rwork	0.217
R-free	0.25634
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vh5
RMSD bond length	0.007
RMSD bond angle	1.442
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.850
High resolution limit [Å]	2.790	7.590	2.800
Rmerge	0.165	0.057	1.785
Rmeas	0.181	0.064	1.954
Rpim	0.073	0.027	0.788
Number of reflections	36360	1863	1842
<I/σ(I)>	6
Completeness [%]	99.9	99.4	100
Redundancy	6	5.2	5.8
CC(1/2)	0.990	0.996	0.639

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.5	289	100mM BIS-TRIS pH 5.5, 25% PEG 3350