8YOQ

Crystal structure of hFSP1 with both 6-OH-FAD and NADP (hFSP1-6-OH-FAD-NADP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.987
Spacegroup name	P 32 2 1
Unit cell lengths	60.302, 60.302, 194.721
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.450 - 1.790
R-factor	0.1957
Rwork	0.194
R-free	0.21870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8yox
RMSD bond length	0.008
RMSD bond angle	0.927
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.021	1.806
High resolution limit [Å]	1.780	1.800
Rmeas	0.301
Rpim	0.069
Number of reflections	40126	3915
<I/σ(I)>	19.6
Completeness [%]	94.3
Redundancy	18.9
CC(1/2)	0.997	0.704

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.7	291.15	0.1 M Sodium chloride, 0.12 M Lithium sulfate, 0.1 M Sodium HEPES, 16.5% PEG 6000