8YD7

Structure of FADD/Caspase-8/cFLIP death effector domain assembly

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-27
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97939
Spacegroup name	P 21 21 21
Unit cell lengths	113.936, 150.057, 175.703
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.810 - 3.320
R-factor	0.1957
Rwork	0.194
R-free	0.23110
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.428
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	3.440
High resolution limit [Å]	3.320	7.120	3.320
Rmerge	0.124	0.076	0.554
Rmeas		0.084	0.582
Rpim		0.033	0.175
Number of reflections	44899	4584	4440
<I/σ(I)>	20.228
Completeness [%]	99.5	96.2	100
Redundancy	9.6	6.5	10.7
CC(1/2)		0.993	0.955

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	HEPES, TBG, PEG8000, TCEP, sodium chloride