8Y6F

The crystal structure of MMPs cleavable human heavy chain ferritin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-30
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9798
Spacegroup name	P 42 21 2
Unit cell lengths	219.660, 219.660, 148.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.520 - 2.010
R-factor	0.2468
Rwork	0.245
R-free	0.28670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.901
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.470	2.080
High resolution limit [Å]	2.010	2.010
Rmerge	0.155	0.688
Number of reflections	237253	23455
<I/σ(I)>	8.68
Completeness [%]	99.0	99.43
Redundancy	7.7
CC(1/2)	0.998	0.909

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	285	0.1 M Tris 8.0, 1% PEG 8000, 20% isopropanol