Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2022-05-17 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5870 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 210.105, 56.922, 65.066 |
| Unit cell angles | 90.00, 107.47, 90.00 |
Refinement procedure
| Resolution | 46.381 - 2.792 |
| R-factor | 0.1973 |
| Rwork | 0.194 |
| R-free | 0.25970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7wcl |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.766 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.381 | 2.892 |
| High resolution limit [Å] | 2.792 | 2.792 |
| Rmeas | 0.225 | |
| Number of reflections | 17936 | 1467 |
| <I/σ(I)> | 8 | 2.9 |
| Completeness [%] | 96.9 | 80.08 |
| Redundancy | 6.7 | |
| CC(1/2) | 0.986 | 0.791 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 18% PEG 8000, 0.2 M Li2SO4, and 0.1 M MES, pH 6.5 |
