8XV4
Crystal structure of TTD-PHD domain of UHRF1 in complex with mStella peptide (residues 85-119)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-26 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 141.713, 72.365, 63.065 |
| Unit cell angles | 90.00, 108.05, 90.00 |
Refinement procedure
| Resolution | 19.990 - 3.200 |
| R-factor | 0.22833 |
| Rwork | 0.227 |
| R-free | 0.26563 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.055 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.990 | 3.460 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.166 | 0.447 |
| Rmeas | 0.209 | 0.576 |
| Rpim | 0.126 | 0.358 |
| Total number of observations | 23901 | 4512 |
| Number of reflections | 9681 | 1984 |
| <I/σ(I)> | 5.7 | 2.6 |
| Completeness [%] | 95.8 | |
| Redundancy | 2.5 | 2.3 |
| CC(1/2) | 0.968 | 0.784 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.6 M NaCl, 0.1 M MES pH6.5, 20% PEG4000 |
