8XOQ

Human Calcium and Integrin Binding Protein 2 (CIB2) Fusion to TMC1 CBD-1 domain at 2.4 Angstroms resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97915
Spacegroup name	P 1 21 1
Unit cell lengths	32.065, 121.849, 62.208
Unit cell angles	90.00, 91.74, 90.00

Refinement procedure

Resolution	43.550 - 2.410
R-factor	0.20334
Rwork	0.201
R-free	0.24223
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.258
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.400	6.510	2.400
Rmerge	0.124	0.111	0.263
Rmeas	0.134	0.120	0.289
Rpim	0.052	0.046	0.119
Total number of observations	115387
Number of reflections	18424	939	946
<I/σ(I)>	7
Completeness [%]	99.4	98.6	99.5
Redundancy	6.3	6.8	5.4
CC(1/2)	0.980	0.975	0.948

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	25% (w/v) Polyethylene glycol 3350, 0.1 M Tris (pH 8.5), 0.2 M Magnesium chloride hexahydrate.