8X7N
Cell-cell adhesion Nanobody/Antigen Pair
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 07A |
| Synchrotron site | NSRRC |
| Beamline | TPS 07A |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2023-11-02 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 96.864, 153.263, 163.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.850 - 3.670 |
| R-factor | 0.2615 |
| Rwork | 0.260 |
| R-free | 0.28540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vnv |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.478 |
| Data scaling software | HKL-2000 (v7.22) |
| Phasing software | PHENIX (1.19.2_4158) |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.850 | 3.830 |
| High resolution limit [Å] | 3.670 | 3.670 |
| Number of reflections | 26580 | 2604 |
| <I/σ(I)> | 7.3 | |
| Completeness [%] | 96.5 | |
| Redundancy | 8.6 | |
| CC(1/2) | 0.988 | 0.955 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Sodium acetate pH 4.6, 1.0 M Ammonium sulfate |
