8X7H

Crystal structure of the ternary complex of GID4-PROTAC(NEP162)-BRD4(BD1).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2023-07-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978530
Spacegroup name	P 1
Unit cell lengths	56.855, 87.613, 88.440
Unit cell angles	103.09, 90.90, 106.23

Refinement procedure

Resolution	38.460 - 2.900
R-factor	0.2388
Rwork	0.235
R-free	0.30100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.759
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.810	3.010
High resolution limit [Å]	2.900	2.900
Rmerge	0.090	0.528
Number of reflections	34488	3438
<I/σ(I)>	9.97
Completeness [%]	97.1
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2M potassium sodium tartrate tetrahydrate, 0.1M Bis-Tris pH 6.5, 10% (w/v) polyethylene glycol 10000