8WWZ
Crystal structure of Bacillus subtilis glyceraldehyde-3-phosphate dehydrogenase GapB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 102.600, 102.600, 111.081 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 88.850 - 2.300 |
| R-factor | 0.1963 |
| Rwork | 0.194 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.674 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19rc5_4047: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.850 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.143 | 0.776 |
| Rmeas | 0.143 | 0.797 |
| Rpim | 0.033 | 0.181 |
| Number of reflections | 15937 | 1519 |
| <I/σ(I)> | 16 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 19 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.5 | 293 | 13.4% (v/v) PEG 400, 10.05% (w/v) PEG 1000, 0.15M Potassium phosphate dibasic/Sodium phosphate monobasic pH 6.5 |
