8WWY

Crystal structure of mouse TIFA/TIFAB heterodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 61 2 2
Unit cell lengths	88.758, 88.758, 297.367
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.040 - 1.790
R-factor	0.1833
Rwork	0.181
R-free	0.22480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.818
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.040	1.900
High resolution limit [Å]	1.790	1.790
Number of reflections	65696	10329
<I/σ(I)>	21.4
Completeness [%]	99.7
Redundancy	19.5
CC(1/2)	1.000	0.738

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	HEPES, PEG 2000, Hexanediol