8WWR

Crystal structure of apo human dishevelled 2 (Dvl2) PDZ domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-27
Detector	DECTRIS PILATUS3 X 2M
Wavelength(s)	0.87313
Spacegroup name	P 43 21 2
Unit cell lengths	45.978, 45.978, 78.422
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.510 - 1.750
R-factor	0.2282
Rwork	0.227
R-free	0.25270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.089
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.510	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.067	0.731
Number of reflections	9023	482
<I/σ(I)>	23.3	3.8
Completeness [%]	100.0	100
Redundancy	17.5	15.9
CC(1/2)	0.999	0.915

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M Tri-sodium citrate, pH 5.6 20% (v/v) Isopropanol 20%(w/v) PEG4000