8WUL
Crystal structure of affinity enhanced TCR in complex with HLA-A*11:01 bound to KRAS-G12V peptide (VVGAVGVGK)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.03845 |
| Spacegroup name | P 1 |
| Unit cell lengths | 87.752, 96.331, 123.121 |
| Unit cell angles | 94.56, 90.63, 93.20 |
Refinement procedure
| Resolution | 29.670 - 2.360 |
| Rwork | 0.190 |
| R-free | 0.23480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.493 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.400 |
| High resolution limit [Å] | 2.360 | 2.360 |
| Number of reflections | 161796 | 7989 |
| <I/σ(I)> | 27.1 | 1.7 |
| Completeness [%] | 98.1 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.990 | 0.796 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289.15 | 20% (w/v) PEG 3350, 0.2M ammonium citrate tribasic at pH 7.0 |
