8WHL

Crystal structure of CLASP2 in complex with CENP-E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 61
Unit cell lengths	106.221, 106.221, 327.051
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.890 - 3.200
R-factor	0.2479
Rwork	0.246
R-free	0.27750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.484
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.260
High resolution limit [Å]	3.200	8.670	3.200
Rmerge	0.117	0.070	1.035
Rmeas	0.128	0.077	1.130
Rpim	0.051	0.031	0.449
Total number of observations	217234
Number of reflections	34412	1762	1705
<I/σ(I)>	5.2
Completeness [%]	99.9	99.5	99.9
Redundancy	6.3	6.1	6.3
CC(1/2)	0.991	0.993	0.545

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1 M HEPES pH 6.5 and 1.4 M sodium citrate