8WG1

Crystal structure of GH97 glucodextranase mutant E509Q from Flavobacterium johnsoniae in complex with panose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-25
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	290.693, 102.708, 104.529
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.230 - 2.340
R-factor	0.21057
Rwork	0.208
R-free	0.25445
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	2.078
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.230	2.380
High resolution limit [Å]	2.340	2.340
Rmerge	0.210	1.347
Rmeas	0.227	1.453
Rpim	0.086	0.542
Number of reflections	132638	89573
<I/σ(I)>	11.7	2.1
Completeness [%]	100.0	100
Redundancy	13.2	13.8
CC(1/2)	0.997	0.734

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	12% PEG20000, 200 mM sodium cacodylate buffer (pH 6.0), 200 mM magnesium acetate, 10 mM glucose