8W92

human H ferritin with 2 Fe(II)/subunit loading

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-01
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.6314
Spacegroup name	F 4 3 2
Unit cell lengths	182.220, 182.220, 182.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.200 - 2.120
R-factor	0.1667
Rwork	0.165
R-free	0.20030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.820
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.200	2.196
High resolution limit [Å]	2.120	2.121
Number of reflections	15266	1491
<I/σ(I)>	31.86
Completeness [%]	99.9
Redundancy	28
CC(1/2)	1.000	0.832

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM HEPES, pH7, 20 mM CaCl2