8W78

Structure of Drosophila melanogaster L-2-hydroxyglutarate dehydrogenase in complex with FAD and 2-oxoglutarate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	P 1 21 1
Unit cell lengths	100.082, 102.330, 102.632
Unit cell angles	90.00, 108.33, 90.00

Refinement procedure

Resolution	31.670 - 2.810
R-factor	0.2305
Rwork	0.228
R-free	0.27730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.471
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.870
High resolution limit [Å]	2.810	7.650	2.820
Rmerge	0.174	0.034	1.430
Rmeas	0.189	0.037	1.566
Rpim	0.072	0.014	0.626
Number of reflections	47660	2470	2353
<I/σ(I)>	3.8
Completeness [%]	99.4	99.2	98.6
Redundancy	6.7	6.6	5.9
CC(1/2)	0.994	0.999	0.533

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	0.2 M magnesium formate and 18% (w/v) PEG 3350, 40 mM 2-OG