8W4M

Crystal structure of open conformation of human immunoglobulin Fc in presence of EndoSz

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2021-06-28
Detector	RAYONIX MX300-HS
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	50.088, 147.668, 75.198
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.340 - 2.180
R-factor	0.2103
Rwork	0.208
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.340	2.258
High resolution limit [Å]	2.180	2.180
Number of reflections	14109	787
<I/σ(I)>	5.4
Completeness [%]	94.1
Redundancy	5.4
CC(1/2)	0.925	0.691

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	100 mM MES PH 6.5 200 mM Zinc acetate 10 % PEG 8000