8W0Y

Crystal structure of broadly neutralizing antibody hcab17 in complex with Hepatitis C virus envelope glycoprotein E2 ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	298
Detector technology	PIXEL
Collection date	2021-09-21
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.0
Spacegroup name	P 3 2 1
Unit cell lengths	179.410, 179.410, 168.762
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	77.690 - 3.310
R-factor	0.2115
Rwork	0.209
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.098
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.710	3.430
High resolution limit [Å]	3.310	3.310
Rmerge	0.483	3.032
Rmeas	0.510	3.216
Rpim	0.163	1.063
Total number of observations	459905	40627
Number of reflections	47162	4577
<I/σ(I)>	3.7	1.1
Completeness [%]	100.0
Redundancy	9.8	8.9
CC(1/2)	0.970	0.235

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	1.0 M Sodium citrate tribasic dihydrate and 0.1 M Sodium cacodylate