8VZZ

Q108K:K40L:T51V:T53S:Y19W:R58W mutant of hCRBPII bound to synthetic fluorophore TD-1V

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-24
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97872
Spacegroup name	P 1
Unit cell lengths	29.329, 36.432, 64.083
Unit cell angles	89.74, 89.36, 66.38

Refinement procedure

Resolution	29.600 - 1.220
Rwork	0.179
R-free	0.18300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qyn
RMSD bond length	0.011
RMSD bond angle	1.447
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.16)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.600	1.290
High resolution limit [Å]	1.220	1.220
Number of reflections	72294	6768
<I/σ(I)>	44.63
Completeness [%]	93.5
Redundancy	13.5
CC(1/2)	0.889	0.902

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	PEG4000, ammonium acetate, sodium acetate