8VTW
Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with macrolone MCX-128 and protein Y at 2.35A resolution
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-24 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 209.600, 449.320, 619.650 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 124.580 - 2.350 |
R-factor | 0.2087 |
Rwork | 0.207 |
R-free | 0.24290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6xhx |
RMSD bond length | 0.005 |
RMSD bond angle | 0.901 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 124.580 | 124.580 | 2.410 |
High resolution limit [Å] | 2.350 | 10.510 | 2.350 |
Rmerge | 0.186 | 0.064 | 2.577 |
Rmeas | 0.200 | 0.069 | 2.749 |
Total number of observations | 18790343 | ||
Number of reflections | 2389130 | 27799 | 175929 |
<I/σ(I)> | 8.67 | 28.41 | 0.88 |
Completeness [%] | 100.0 | 99.1 | 99.9 |
Redundancy | 7.865 | 7.847 | 8.011 |
CC(1/2) | 0.993 | 0.992 | 0.182 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.6 | 292 | 0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME |