8VME

Crystal structure of the GSK-3/Axin complex bound to a phosphorylated beta-catenin T41A peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-21
Detector	PSI PILATUS 6M
Wavelength(s)	0.97949
Spacegroup name	P 61 2 2
Unit cell lengths	82.257, 82.257, 280.990
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.470 - 2.300
R-factor	0.1828
R-free	0.22980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nm7
RMSD bond length	0.010
RMSD bond angle	1.164
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.470	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.152	5.635
Rmeas	0.154	5.768
Rpim	0.026	1.201
Number of reflections	26090	2435
<I/σ(I)>	17.1	0.8
Completeness [%]	99.9	98.8
Redundancy	35.5	21.8
CC(1/2)	1.000	0.399

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	7.5	277	10% PEG35000, 20 mM Tris, pH 7.5, 300 mM sodium chloride, 5% glycerol, 10 mM magnesium chloride, 200 uM ATP, 5 mM DTT