8VLD
Crystal structure of Ash1L PHD finger in complex with histone H3K4me2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-03 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.28 |
| Spacegroup name | P 43 |
| Unit cell lengths | 49.960, 49.960, 46.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.330 - 1.920 |
| R-factor | 0.2034 |
| Rwork | 0.201 |
| R-free | 0.24910 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.911 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.330 | 1.990 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.060 | 0.096 |
| Number of reflections | 16874 | 849 |
| <I/σ(I)> | 31 | 12.3 |
| Completeness [%] | 99.8 | |
| Redundancy | 18 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 1.6 M sodium citrate pH 6.5 |
