8VJ2

Crystal Structure of Macrophage migration inhibitory factor-1 (MIF1) from Onchocerca volvulus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 19-ID
Synchrotron site	NSLS-II
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-09
Detector	DECTRIS EIGER2 XE 9M
Wavelength(s)	0.9785
Spacegroup name	C 2 2 21
Unit cell lengths	52.441, 91.127, 132.487
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.450 - 1.900
R-factor	0.2176
Rwork	0.217
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.700
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.21rc1_5177: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.450	1.950
High resolution limit [Å]	1.900	1.900
Rmerge	0.087	1.095
Rmeas	0.091	1.136
Rpim	0.025	0.299
Total number of observations	340455	25834
Number of reflections	25433	1843
<I/σ(I)>	15	2.1
Completeness [%]	99.9
Redundancy	13.4	14
CC(1/2)	0.999	0.956

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	Berkeley B3: 100 mM Bis-Tris / Hydrochloric acid pH 6.5 400 mM Sodium chloride 30% (w/v) PEG 3350, OnvoA.00834.a.UX1.PW39231 at 20.1 mg/mL. plate 13715 well B3 drop 2. Puck: PSL-1003, Cryo: crystallization solution