8VFA
Ternary DNA Polymerase Beta bound to DNA containing primer terminal dC base-paired with FapydG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-05-29 |
Detector | DECTRIS PILATUS3 R 200K-A |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.437, 79.531, 54.889 |
Unit cell angles | 90.00, 108.26, 90.00 |
Refinement procedure
Resolution | 24.940 - 2.050 |
R-factor | 0.2286 |
Rwork | 0.226 |
R-free | 0.26670 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.423 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.090 |
High resolution limit [Å] | 2.050 | 2.050 |
Number of reflections | 38424 | 1274 |
<I/σ(I)> | 24.7 | |
Completeness [%] | 99.1 | |
Redundancy | 4.6 | |
CC(1/2) | 0.999 | 0.828 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 14-22% PEG 3350 0.05 M Imidazole pH 8.0 0.35 M Sodium Acetate |