8UVW

Crystal structure of RAD51-BRCA2 Cter complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	51.463, 98.551, 128.324
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.820 - 2.730
R-factor	0.2496
Rwork	0.248
R-free	0.28210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.467
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.21rc1_4924:000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.240	2.860
High resolution limit [Å]	2.730	2.730
Number of reflections	17974	2352
<I/σ(I)>	15.9	0.6
Completeness [%]	99.8	99.9
Redundancy	12.7	13.6
CC(1/2)	0.999	0.340

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	25% v/v 1,4-Dioxane 0.05 M MOPS pH 7.0 0.005 M Magnesium chloride hexahydrate 0.001 M Spermine