8UVS

Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with spectinomycin derivative 2694, mRNA, deacylated A- and E-site tRNAphe, and deacylated P-site tRNAmet at 2.75A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-09
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97920
Spacegroup name	P 21 21 21
Unit cell lengths	209.730, 449.080, 621.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	121.840 - 2.750
R-factor	0.2154
Rwork	0.213
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xhw
RMSD bond length	0.004
RMSD bond angle	0.836
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	121.840	121.840	2.820
High resolution limit [Å]	2.750	12.300	2.750
Rmerge	0.151	0.029	1.588
Rmeas	0.179	0.035	1.870
Total number of observations	5085095
Number of reflections	1483558	16526	109503
<I/σ(I)>	7.42	30.95	0.87
Completeness [%]	98.9	93.1	99.1
Redundancy	3.428	3.316	3.467
CC(1/2)	0.993	0.997	0.208

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME