8UVS

Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with spectinomycin derivative 2694, mRNA, deacylated A- and E-site tRNAphe, and deacylated P-site tRNAmet at 2.75A resolution

This is a non-PDB format compatible entry.

Summary for 8UVS

• Entry DOI: 10.2210/pdb8uvs/pdb
• Descriptor: 23S Ribosomal RNA, 50S ribosomal protein L14, 50S ribosomal protein L15, ... (61 entities in total)
• Functional Keywords: spectinomycin; aminomethyl spectinomycin; antibiotic; 70s ribosome; x-ray structure; inhibition of translation; infectious diseases; antimicrobial drug discovery; semisynthetic chemistry; drug development, ribosome, ribosome-inhibitor complex, ribosome/inhibitor
• Biological source: Escherichia coli; More
• Total number of polymer chains: 112
• Total formula weight: 4572867.66

Authors

Killam, B.Y.,Phelps, G.A.,Lee, R.E.,Polikanov, Y.S. (deposition date: 2023-11-03, release date: 2024-08-07)

• Primary citation: Phelps, G.A.,Cheramie, M.N.,Fernando, D.M.,Selchow, P.,Meyer, C.J.,Waidyarachchi, S.L.,Dharuman, S.,Liu, J.,Meuli, M.,Molin, M.D.,Killam, B.Y.,Murphy, P.A.,Reeve, S.M.,Wilt, L.A.,Anderson, S.M.,Yang, L.,Lee, R.B.,Temrikar, Z.H.,Lukka, P.B.,Meibohm, B.,Polikanov, Y.S.,Hobbie, S.N.,Bottger, E.C.,Sander, P.,Lee, R.E.; Development of 2nd generation aminomethyl spectinomycins that overcome native efflux in Mycobacterium abscessus.; Proc.Natl.Acad.Sci.USA, 121:e2314101120-e2314101120, 2024; PubMed: 38165935; DOI: 10.1073/pnas.2314101120

Experimental method

X-RAY DIFFRACTION (2.75 Å)