8URF

Crystal Structure of human ASGR2 CRD (Carbohydrate Recognition Domain) bound to 8G8 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.000
Spacegroup name	H 3 2
Unit cell lengths	102.410, 102.410, 358.985
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.544 - 1.900
R-factor	0.1679
Rwork	0.166
R-free	0.20350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.225
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.14rc1_3177: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.900	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.107	3.193
Rmeas	0.109	3.273
Rpim	0.025	0.718
Number of reflections	57655	3681
<I/σ(I)>	16.3	1.2
Completeness [%]	100.0	100
Redundancy	19.8	20.7
CC(1/2)	0.999	0.440

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	100mM sodium HEPES pH 7.5, 100mM calcium chloride, 30% (v/v) PEG400 Cryo prodtecion with 16% (v/v) glycerol