8UQR

Crystal structure of the human p53 tetramerization domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE 7B2
Synchrotron site	CHESS
Beamline	7B2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9686
Spacegroup name	P 21 21 21
Unit cell lengths	30.973, 56.437, 61.656
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.630 - 1.220
R-factor	0.1461
Rwork	0.144
R-free	0.17670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.024
RMSD bond angle	1.763
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.630	1.264
High resolution limit [Å]	1.220	1.220
Rmerge	0.045
Number of reflections	30355	1953
<I/σ(I)>	19.68
Completeness [%]	91.8
Redundancy	5.5
CC(1/2)	1.000	0.959

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	15% PEG 1500