8UQE
Crystal structure of RNF168 (RING)-UbcH5c fused to H2A-H2B via a 26-residue linker (RING not modeled in density)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-11-28 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 109.279, 109.279, 86.782 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.540 - 3.562 |
R-factor | 0.251 |
Rwork | 0.249 |
R-free | 0.26760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5egg |
RMSD bond length | 0.003 |
RMSD bond angle | 0.501 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.620 |
High resolution limit [Å] | 3.562 | 3.562 |
Rmerge | 0.118 | 0.705 |
Rmeas | 0.128 | 0.761 |
Rpim | 0.048 | 0.281 |
Number of reflections | 7433 | 720 |
<I/σ(I)> | 13 | 2.46 |
Completeness [%] | 99.3 | 98.61 |
Redundancy | 6.8 | 6.7 |
CC(1/2) | 0.999 | 0.901 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 288 | Crystals were obtained by mixing 2 microliters of the protein fusion (10 mg/mL) in 10 mM HEPES, pH 7.5, 600 mM NaCl, 1 mM TCEP and 2 microliters of the reservoir solution containing 0.1 M imidazole, pH 6.5, 1.0 M sodium acetate |