8UQC

Crystal structure of RNF168 (RING)-UbcH5c fused to H2A-H2B via a 20-residue linker (crystallization condition 2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2018-11-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 32 2 1
Unit cell lengths	101.981, 101.981, 86.569
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.940 - 2.610
R-factor	0.222
Rwork	0.219
R-free	0.25210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gb0 5egg
RMSD bond length	0.002
RMSD bond angle	0.495
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.940	2.703
High resolution limit [Å]	2.610	2.610
Rmerge	0.140	0.863
Rmeas	0.150	0.930
Rpim	0.054	0.340
Number of reflections	16208	1576
<I/σ(I)>	14.99	2.57
Completeness [%]	99.9	99.68
Redundancy	7.7	7.6
CC(1/2)	0.996	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	288	Crystals were obtained by mixing 2 microliters of the protein fusion (12 mg/mL) in 10 mM HEPES, pH 7.5, 600 mM NaCl, 1 mM TCEP and 2 microliters of the reservoir solution containing 4% tacsimate, pH 6, 13.5% PEG3350