8UQB
Crystal structure of RNF168 (RING)-UbcH5c fused to H2A-H2B via a 20-residue linker (crystallization condition 1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-02-02 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 101.948, 101.948, 86.992 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.370 - 2.484 |
R-factor | 0.2223 |
Rwork | 0.218 |
R-free | 0.25960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4gb0 5egg |
RMSD bond length | 0.004 |
RMSD bond angle | 0.656 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.573 |
High resolution limit [Å] | 2.484 | 2.484 |
Rmerge | 0.113 | 0.777 |
Rmeas | 0.126 | 0.877 |
Rpim | 0.053 | 0.396 |
Number of reflections | 18662 | 1718 |
<I/σ(I)> | 16.39 | 2.66 |
Completeness [%] | 99.4 | 94.24 |
Redundancy | 5.3 | 4.6 |
CC(1/2) | 0.996 | 0.731 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 288 | Crystals were obtained by mixing 2 microliters of the protein fusion (12 mg/mL) in 10 mM HEPES, pH 7.5, 600 mM NaCl, 1 mM TCEP and 2 microliters of the reservoir solution containing 50 mM BIS-TRIS propane, pH 7.0, 1 M NaCl |