8UO7

Bovine trypsin in complex with deacetylated wild type microviridin J

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9201
Spacegroup name	P 32 2 1
Unit cell lengths	71.650, 71.650, 72.976
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.550 - 2.200
R-factor	0.19944
Rwork	0.197
R-free	0.25818
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	REFMAC (8.0.013)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.550	2.279
High resolution limit [Å]	2.200	2.200
Rmerge	0.190	0.874
Rmeas	0.201	0.923
Number of reflections	11366	1120
<I/σ(I)>	8.34
Completeness [%]	99.9	99.91
Redundancy	9.8
CC(1/2)	0.991	0.883

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295.37	0.1 M sodium cacodylate pH 7.0, 0.2 M ammonium sulfate, 30%(w/v) PEG 8000