8UNI

LSD1-CoREST with N-formyl-FAD in complex with H3K4M histone tail

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-02
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.999998
Spacegroup name	I 2 2 2
Unit cell lengths	117.782, 178.172, 234.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.130 - 3.400
R-factor	0.2233
Rwork	0.222
R-free	0.25240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.476
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.130	3.570
High resolution limit [Å]	3.400	3.400
Rmerge	0.281	3.254
Rmeas	0.305	3.512
Rpim	0.116	1.311
Total number of observations	233960	31984
Number of reflections	34222	4507
<I/σ(I)>	5.8	0.7
Completeness [%]	99.8
Redundancy	6.8	7.1
CC(1/2)	0.994	0.495

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.2 M Sodium Potassium Tartrate, 100 mM ADA pH 6.5