8ULK

Prefusion RSV F bound by neutralizing antibody 1G12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	143.982, 174.605, 226.286
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.950 - 4.280
R-factor	0.246
Rwork	0.244
R-free	0.27860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond angle	5.079
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.950	4.430
High resolution limit [Å]	4.280	4.280
Number of reflections	36919	1605
<I/σ(I)>	7.2
Completeness [%]	72.9
Redundancy	7.3
CC(1/2)	0.940	0.640

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.2 M Sodium sulfate, 20 % w/v PEG 3350