8UFY
High Resolution structure of A. viridans Lactate Oxidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-03-21 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.7293 |
Spacegroup name | I 4 |
Unit cell lengths | 132.262, 132.262, 91.680 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.700 - 1.200 |
R-factor | 0.1467 |
Rwork | 0.146 |
R-free | 0.16190 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.805 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.21rc1_5084) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.350 | 1.190 |
High resolution limit [Å] | 1.170 | 1.170 |
Rpim | 0.040 | 0.801 |
Number of reflections | 517344 | 13078 |
<I/σ(I)> | 9.7 | 1.1 |
Completeness [%] | 99.9 | 100 |
Redundancy | 4.7 | |
CC(1/2) | 0.998 | 0.349 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 26-30.5% ETHYLENE GLYCOL, 100 mM MES ph 4.0, 125mM sodium Lactate |