8U9L

Crystal Structure of RelA-cRel chimera complex with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 1
Unit cell lengths	65.910, 110.464, 162.836
Unit cell angles	82.20, 77.98, 72.81

Refinement procedure

Resolution	47.922 - 3.091
R-factor	0.2781
Rwork	0.277
R-free	0.32890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.349
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.16_3549: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.210
High resolution limit [Å]	3.091	6.670	3.100
Rmerge	0.089	0.043	0.496
Rmeas	0.126	0.060	0.701
Rpim	0.089	0.043	0.496
Number of reflections	76211	7663	7644
<I/σ(I)>	7.3
Completeness [%]	98.3	98.7	98.5
Redundancy	1.9	2	1.9
CC(1/2)	0.990	0.995	0.830

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2M Sodium/Potassium phosphate 0.1M Bis Tris propane pH 6.5 20% PEG 3350 10mM DTT 0.5% BOG