8U1G
Prefusion-stabilized SARS-CoV-2 S2 subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | H 3 |
| Unit cell lengths | 78.990, 78.990, 479.530 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 67.720 - 3.200 |
| R-factor | 0.2357 |
| Rwork | 0.226 |
| R-free | 0.26190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.561 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.920 | 3.420 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 18384 | 3362 |
| <I/σ(I)> | 3.2 | 1.8 |
| Completeness [%] | 99.7 | |
| Redundancy | 4.4 | |
| CC(1/2) | 0.850 | 0.520 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES pH 7.5, 8% (v/v) ethylene glycol and 10% (v/v) PEG 8000 |
