8U0O

Synaptic complex of human DNA polymerase Lambda DL variant engaged on a DNA double-strand break containing an unpaired 3' primer terminus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	93
Detector technology	PIXEL
Collection date	2021-11-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 32 2 1
Unit cell lengths	58.971, 58.971, 211.248
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.340 - 2.050
R-factor	0.217
Rwork	0.215
R-free	0.25860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.167
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.090
High resolution limit [Å]	2.050	2.050
Rmeas	0.078	0.931
Rpim	0.025	0.313
Number of reflections	27862	1349
<I/σ(I)>	30.92	1.25
Completeness [%]	99.9	99.3
Redundancy	9.6	7.9
CC(1/2)	0.993	0.804

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	294	0.1 M ADA pH 6.5, 1 M ammonium phosphate dibasic