8TZI

Human equilibrative nucleoside transporter-1, JH-ENT-01 bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2019-07-06
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 32 2 1
Unit cell lengths	72.413, 72.413, 173.920
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	58.990 - 2.700
R-factor	0.226
Rwork	0.229
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ob7
RMSD bond length	0.003
RMSD bond angle	0.544
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.710	2.830
High resolution limit [Å]	2.700	2.700
Number of reflections	15151	1945
<I/σ(I)>	3.9
Completeness [%]	99.8
Redundancy	6.9
CC(1/2)	0.970	0.328

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	9	298	35-50% PEG400, 0.1 M glycine, 0.5 M sodium chloride