8TZI
Human equilibrative nucleoside transporter-1, JH-ENT-01 bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-07-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 72.413, 72.413, 173.920 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 58.990 - 2.700 |
R-factor | 0.226 |
Rwork | 0.229 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ob7 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.544 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX (1.20.1_4487) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.710 | 2.830 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 15151 | 1945 |
<I/σ(I)> | 3.9 | |
Completeness [%] | 99.8 | |
Redundancy | 6.9 | |
CC(1/2) | 0.970 | 0.328 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 9 | 298 | 35-50% PEG400, 0.1 M glycine, 0.5 M sodium chloride |