8TX5
Crystal structure of an engineered variant of galactose oxidase, GOaseRd4BB, from Fusarium graminearum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-08-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 89.010, 205.753, 89.291 |
Unit cell angles | 90.00, 92.93, 90.00 |
Refinement procedure
Resolution | 28.020 - 1.930 |
R-factor | 0.1649 |
Rwork | 0.163 |
R-free | 0.19800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.018 |
RMSD bond angle | 1.408 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | BUSTER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.020 | 2.190 |
High resolution limit [Å] | 1.930 | 2.080 |
Number of reflections | 223522 | 27814 |
<I/σ(I)> | 9.8 | |
Completeness [%] | 92.7 | |
Redundancy | 3.5 | |
CC(1/2) | 0.996 | 0.810 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 10% vol PEG 3350, 200 mM Potassium monophosphate, 100 mM TRIS.HCl (pH 8.0) |