8TV1
Structure of the EphA2 LBDCRD bound to FabS1C_L1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.437, 73.249, 118.570 |
| Unit cell angles | 90.00, 107.43, 90.00 |
Refinement procedure
| Resolution | 62.200 - 2.600 |
| R-factor | 0.2048 |
| Rwork | 0.202 |
| R-free | 0.24620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.040 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.200 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.130 | 1.102 |
| Rmeas | 0.149 | 1.269 |
| Rpim | 0.072 | 0.619 |
| Number of reflections | 28513 | 3018 |
| <I/σ(I)> | 6.2 | 1.7 |
| Completeness [%] | 91.4 | 79.4 |
| Redundancy | 3.9 | 3.8 |
| CC(1/2) | 0.989 | 0.612 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 14% PEG 20K, 100 mm HEPES 7.0, 200 mM KSCN |
