8TUD

IgG1 Fc Heterodimer combYSelect2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	50.014, 79.685, 139.308
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.360 - 3.000
R-factor	0.2063
Rwork	0.198
R-free	0.28520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.221
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PDB-REDO

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.360	3.108
High resolution limit [Å]	3.000	3.000
Rmerge	0.453	3.074
Rmeas	0.474	3.221
Rpim	0.137	0.949
Number of reflections	11350	993
<I/σ(I)>	18.5	2.7
Completeness [%]	96.9	86.27
Redundancy	11.5	10.7
CC(1/2)	0.964	0.628

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	292	0.1M Bis Tris pH 6.5 and 29% PEG MME 2000