8TUB
HLA B7:02 with HPNGYKSLSTL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.211, 90.668, 104.563 |
| Unit cell angles | 97.54, 93.83, 90.23 |
Refinement procedure
| Resolution | 43.000 - 2.400 |
| R-factor | 0.1993 |
| Rwork | 0.197 |
| R-free | 0.23760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.187 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.400 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rpim | 0.130 | 0.343 |
| Number of reflections | 66085 | 6521 |
| <I/σ(I)> | 6.49 | |
| Completeness [%] | 94.5 | 93.1 |
| Redundancy | 2.8 | |
| CC(1/2) | 0.953 | 0.706 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG |
