8TTM

IgG1 Fc Heterodimer combYSelect1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	49.782, 79.394, 142.902
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.880 - 2.510
R-factor	0.20601
Rwork	0.202
R-free	0.24250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.446
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PDB-REDO

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.880	2.600
High resolution limit [Å]	2.510	2.510
Rmerge	0.240	0.961
Rmeas	0.254	1.020
Rpim	0.079	0.336
Number of reflections	19700	1814
<I/σ(I)>	30.3	2.25
Completeness [%]	98.1
Redundancy	10.2
CC(1/2)	0.993	0.852

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	292	0.1M BisTris pH 6.5 and 21% PEG MME 5000