8TRV

Structure of the EphA2 LBDCRD bound to FabS1C_C1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 2 21
Unit cell lengths	73.933, 110.558, 263.443
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	131.720 - 3.250
R-factor	0.2197
Rwork	0.217
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 3FL7 & 8TRS
RMSD bond length	0.010
RMSD bond angle	1.161
Data reduction software	X-Area
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	131.720	3.100
High resolution limit [Å]	2.990	2.990
Rmerge	0.121	2.214
Rmeas	0.132	2.427
Rpim	0.050	0.975
Number of reflections	44485	4523
<I/σ(I)>	12	0.9
Completeness [%]	99.6	98.6
Redundancy	6.7	5.9
CC(1/2)	0.999	0.349

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	20% PEG3350, 200 mM sodium iodide (PACT Premiere solution E3)