8TLR

Crystal Structure of human HRAS G12C covalently bound to AMG 510

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	77.36
Detector technology	CCD
Collection date	2022-10-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	H 3 2
Unit cell lengths	92.449, 92.449, 119.711
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.965 - 1.700
R-factor	0.185069278418
Rwork	0.184
R-free	0.20980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.273
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHENIX (1.10-1-2155)
Refinement software	PHENIX (1.10-1-2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.550	1.730
High resolution limit [Å]	1.700	1.700
Number of reflections	21565	1132
<I/σ(I)>	15.6	6.7
Completeness [%]	99.0
Redundancy	4.8
CC(1/2)	0.976	0.569

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1 M MES 30% PEG 4000